Genetic engineering is changing our lives profoundly. It enables us to design new species, produce biological products and treat diseases by modifying genes. And since genetic engineering is on its way to our life, getting to know it is our priority now. So, let us open this door of mystery together.
开设学校:昆明理工大学;学科:理学、
Genetic engineering is changing our lives profoundly. It enables us to design new species, produce biological products and treat diseases by modifying genes. And since genetic engineering is on its way to our life, getting to know it is our priority now. So, let us open this door of mystery together.
-1.1 Introduction and Basic Concepts - What Makes the Blue Blooms of Roses - Let Us Start to Learn about the Genetic Engineering
-1.2 History - How Has Genetic Engineering Come into Being
--1.2 History - How Has Genetic Engineering Come into Being
-1.3 Basis of Molecular Biology
--1.3.2 Basis of Molecular Biology 2 - Why Do Children Look Like Parents - Gene Expression
-Chapter 1 Homework
-2.1 Nucleic Acid Extraction - How to Get DNA in an Organism - Nucleic Acid Extraction Tells You the Truth
-2.2 Nucleic Acid Detection - How to Confirm Whether DNA Has Been Obtained
--2.2 Nucleic Acid Detection - How to Confirm Whether DNA Has Been Obtained
-2.3 PCR Principle - A Good Way to Increase DNA
--2.3 PCR Principle - A Good Way to Increase DNA
-2.4 PCR Influencing Factors - Increase DNA by Experiments
--2.4 PCR Influencing Factors - Increase DNA by Experiments
-2.5 Reverse Transcription PCR and the Construction of cDNA Library
--2.5 Reverse Transcription PCR and the Construction of cDNA Library
-2.6 The Genome-Wide Amplification and Genome Research
--2.6 The Genome-Wide Amplification and Genome Research
-2.7 Tool Enzyme - Scissors and Glue in Genetic Engineering - Tool Enzyme
--2.7 Tool Enzyme - Scissors and Glue in Genetic Engineering - Tool Enzyme
-Chapter 2 Homework
-3.1 Blue-White Screening - Fun Blue-White Screening - Strain Screening Method Based on Colors
--3.1 Blue-White Screening - Fun Blue-White Screening - Strain Screening Method Based on Colors
-3.2 Plasmid Vector - Who Helps the Scientists Complete the Genetic Modification
--3.2 Plasmid Vector - Who Helps the Scientists Complete the Genetic Modification
-3.3 Viral Vector - Introduce more DNA into Plasmid Vectors to Prepare for Later Sequencing
--3.3 Viral Vector - Introduce more DNA into Plasmid Vectors to Prepare for Later Sequencing
-Chapter 3 Homework
-4.1 Recipient Cells - Selfless Cells in Organisms
--4.1 Recipient Cells - Selfless Cells in Organisms
-4.2 Transformation - The Way to Introduce Selected Genes into Cells
--4.2 Transformation - The Way to Introduce Selected Genes into Cells
-4.3 Screening and Identification of Recombiner - Screening and Identification of Specially Processed Substances
-4.4 DNA Sequencing Technology
--4.4 DNA Sequencing Technology
-Chapter 4 Homework
-5.1 Prokaryotic Expression System
--5.1 Prokaryotic Expression System
-5.2 Eukaryotic Expression System
--5.2 Eukaryotic Expression System
-Chapter 5 Homework
-6.1 Application of Genetic Engineering-Industrial
--6.1.2 Massive Production of Vitamin C by Genetic Recombination Technology
-6.2 Application of Genetic Engineering-Agriculture
--6.2.1 Golden Rice with Vitamin A - How Do We Produce Golden Rice?
--6.2.3 Transgenic Plants and Herbicide Resistance - A Plant that Prevents Weeds from Growing
-6.3 Application of Genetic Engineering-Medicine
--6.3.1 Recombined Vaccine by Gene Engineering - Hepatitis B Vaccine
-6.4 Gene Editing - A Technology that Can Treat Diseases
--6.4 Gene Editing - A Technology that Can Treat Diseases
-Chapter 6 Homework
-7.1 Discussions on Genetic Engineering and Biosecurity - Genetic Engineering Is a Double-Edged Sword
--7.1 Discussions on Genetic Engineering and Biosecurity - Genetic Engineering Is a Double-Edged Sword
-Chapter 7 Homework
-Experiment 1 Extraction and Detection of Genomic DNA of Escherichia Coli
--Experiment 1 Extraction and Detection of Genomic DNA of Escherichia Coli
-Experiment 2 PCR Amplification, Capillary Electrophoresis and Gel Extraction of Chorismate Lyase Gene
--Experiment 3 Cloning, Ligation and Transformation of Genes
-Experiment 3 Cloning, Ligation and Transformation of Genes
--Experiment 3 Cloning, Ligation and Transformation of Genes
-Experiment 4 Plasmid Extraction and Positive Clone Detection
--Experiment 4 Plasmid Extraction and Positive Clone Detection
-Chapter 8 Homework
课程负责人柳陈坚,博士,教授,已从事本科生及研究生教学十余年,具有丰富的教学经验。担任《分子生物学》、《基因工程》、《生物工程专业导论》、《食品生物技术》等课程的教学任务,受到学生和同事的广泛好评。
课程主讲老师李晓然,微生物学博士,副教授,担任《分子生物学》、《基因工程》、《生物工程专业导论》、研究生《专业英语》等课程主讲,连续主讲了六年《基因工程》,具有丰富的教学经验,五年来,学生无记名问卷调查评分均在90分以上。
杨恩,植物学博士,讲师,担任《基因工程》、《生物检测技术》、《生物检测技术实验》、《遗传学》等课程主讲,主讲《基因工程》三年,教学经验丰富,教学态度认真,受到学生的一致好评。
罗义勇,微生物学博士,副教授,已从事本科和研究生教学近10年,具有丰富教学经验。承担本科生《微生物学》、《微生物学实验》和《基因工程 》等本科教学工作以及硕士研究生《基因操作原理和应用》、《应用真菌学》等课程教学任务,获得学生和同事优秀评价。
冯阳 农学博士,担任《生物检测技术》《分子生物学》等课程主讲。
潘卉 遗传学博士,担任《细胞生物学》和《生物化学》课程主讲。