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当前课程知识点:Food Microbiology Testing >  Chapter 6 Detection of Staphylococcus aureus >  6.3 The MPN method >  6.3 The MPN method

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6.3 The MPN method在线视频

下一节:Supporting document

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6.3 The MPN method课程教案、知识点、字幕

最后我们来看金黄色葡萄球菌检测第三法

MPN法

检验的流程包括

样品的处理 接种与培养

鉴定试验 查MPN 表 结果报告

其中

样品的处理和稀释操作与第二法相同

在超净工作台内

选择3个适宜稀释度的样品匀液

每个稀释度分别接种1mL样品

匀液至7.5%氯化钠肉汤管中

每个稀释度接种3管

同时接种1ml生理盐水至

7.5%氯化钠肉汤管中

作为阴性对照

如接种量超过1mL

则用双料7.5%氯化钠肉汤

将上述接种物放在36±1℃条件下培养18-24小时

用一次性接种环从培养后的

7.5%氯化钠肉汤管中分别取培养物1环

移种于Baird-Parker平板

在36±1℃条件下24h~48 小时

将培养后的 Baird-Parker 平板从培养箱中拿出

观察菌落特征

金黄色葡萄球菌在 Baird-Parker 平板上

菌落直径为2-3 毫米

颜色呈灰色黑至黑色

常有浅色的边缘 周围绕以不透明圈

其外常有一清晰带

检样时如果有上述典型或可疑单菌落出现时

进行鉴定试验 与第一法相同

分别进行革兰氏染色

血平板溶血测试和血浆凝固酶实验

根据出现阳性结果

对应的7.5%氯化钠肉汤

三梯度的管数

查阅 MPN 表

具体的查阅方式可以回看

大肠菌群的检测那一节

关于金黄色葡萄球菌检验的课程到这里就结束了

感谢同学们的观看

Food Microbiology Testing课程列表:

Chapter 1 Introduction

-1.1 Introduction

--1.1 Introduction

-Supporting document

-Chapter 1 Discussion

Chapter 2 Routine experimental techniques of microorganism

-2.1 Preparation of culture medium

--2.1 Preparation of culture medium

-2.2 Microbial separation techniques

--2.2 Microbial separation techniques

-2.3 Microbial inoculation techniques

--2.3 Microbial inoculation techniques

-2.4 Exercises

--Chapter 2 exercises

-Supporting document

-Chapter 2 Discussion

Chapter 3 The basic program of food microbiology testing

-3.1 Basic elements of food sample testing

--3.1 Basic elements of food sample testing

-3.2 Sample sampling scheme

--3.2 Sample sampling scheme

-3.3 Sample sampling, transporting, processing, testing and report

--3.3 Sample sampling, transporting, processing, testing and report

-3.4 Exercises

--Chapter 3 exercises

-Supporting document

Chapter 4 Detection of Aerobic Bacterial Count and Coliform in food

-4.1 Detection of Aerobic Bacterial Count

--4.1 Detection of Aerobic Bacterial Count

-4.2 The enumeration of Coliforms

--4.2 The enumeration of Coliforms

-4.3 Exercises

--Chapter 4 exercises

-Supporting document

-Chapter 4 Discussion

Chapter 5 Detection of Salmonella spp.

-5.1 Detection of Salmonella spp. GB4789.4-2016 (Ⅰ)

--5.1 Detection of Salmonella spp. GB4789.4-2016 (Ⅰ)

-5.2 Detection of Salmonella spp. GB4789.4-2016 (Ⅱ)

--5.2 Detection of Salmonella spp. GB4789.4-2016 (Ⅱ)

-5.3 Exercises

--Chapter 5 exercises

-Supporting document

Chapter 6 Detection of Staphylococcus aureus

-6.1 Qualitative method

--6.1 Qualitative method

-6.2 Plate counting

--6.2 Plate counting

-6.3 The MPN method

--6.3 The MPN method

-6.4 Exercises

--Chapter 6 exercises

-Supporting document

Chapter 7 Detection of moulds and yeasts

-7.1 Moulds and yeasts count

--7.1 Moulds and yeasts count

-7.2 Exercises

--Chapter 7 exercises

-Supporting document

Chapter 8 Quality control of food microbiology testing

-8.1 How to control the quality of food microbiology testing

--8.1 How to control the quality of food microbiology testing

-8.2 Quality control of food microbiology testing

--8.2 Quality control of food microbiology testing

-8.3 Requirements of process control

--8.3 Requirements of process control

-8.4 Exercises

--Chapter 8 exercises

-Supporting document

-Chapter 8 Discussion

Chapter 9 Modern food microbiology testing technology

-9.1 Modern food microbiology testing technology (Ⅰ)

--9.1 Modern food microbiology testing technology (Ⅰ)

-9.2 Modern food microbiology testing technology (Ⅱ)

--9.2 Modern food microbiology testing technology (Ⅱ)

-9.3 Exercises

--Chapter 9 exercises

-Supporting document

6.3 The MPN method笔记与讨论

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